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BugBuster® Purification Kits
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Simple extraction of soluble protein from E. coli without sonication BugBuster® Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a detergent mix that is capable of cell wall perforation without denaturing soluble protein.
In practice, cells are harvested by centrifugation and suspended in BugBuster. At this point, Benzonase® Nuclease can be added to reduce the viscosity of the extract due to liberation of chromosomal DNA. Following a brief incubation, insoluble cell debris is removed by centrifugation. The clarified extract is ready to use and fully compatible with the affinity supports offered by Novagen, including GST•Bind™, His•Bind® and S•Tag™ Resins, or other chromatography matrices. Following binding to affinity resin, excess BugBuster is easily removed by washing the column with the appropriate buffer. BugBuster is also useful for the preparation of high purity inclusion bodies in instances where expressed proteins are insoluble. The reagent is available in a variety of configurations.
BugBuster® Purification Kits contain BugBuster, Benzonase, buffers and resins necessary for purification of His•Tag® and GST•Tag™ fusion proteins. |
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