New Release! Organotypic (3D) cultures of rat urothelium.

This 3-D model is generated from the monolayer urothelial cultures described here.

As evident in the H&E stain below, this model forms a 2-3 cell layer thick epithelium, closely matching that found in vivo.

In addition, it has also been characterised and found to express a range of markers, including CK13 and Uroplakin.

A human equivalent of this 3-D model is currently under development.

H&E straining:

Keratin 13 staining (green):

Uroplakin (green):

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