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Isolation of total RNA - TRI Reagent

Why pay much more for TRIzol when you can get an equivalent product TRI Reagent, from the same original manufacturer, Molecular Research Center?

TRI Reagent = TRIzol (TRIzol is a registered trademark of Molecular Research Center, Inc.)
TRI Reagent is a patented reagent based on the single-step method for isolation of RNA . It provides a reliable, cost effective and efficient method for the isolation of total RNA and is the only commercial method available that offers the simultaneous isolation of RNA, DNA and protein from a single sample. TRI Reagent provides the most reliable, cost effective and efficient method for gene expression analysis in a variety of samples.
Cat.No. TR 118
100 ml - $949
200 ml - $1,700
500 ml - $3,700
 
TRI Reagent RT is a NEW reagent which is an upgraded version of an old favorite using the single-step method of RNA isolation. This patent-pending reagent substantially improves the quality of isolated RNA. TRI Reagent RT isolates high purity RNA that is free of DNA contamination, doesn't need DNase treatment and solubilizes more quickly and easily. TRI Reagent RT is the most comprehensive reagent available for analysis of gene expression.

Cat.No. RT 111
100 ml - $1,159
200 ml - $1,934
500 ml - $4,400
 
TRI Reagent RT Protocol

BCP - Phase Separation Reagent
The Phase Separation Reagent consists of molecular biology grade 1-bromo-3-chloropropane (BCP). BCP is a less toxic and more efficient phase-separation agent which replaces chloroform in the single-step method of RNA isolation or the simultaneous isolation of RNA, DNA and proteins. Substituting BCP for chloroform does not affect the quality of isolated RNA, DNA or proteins; however the use of BCP as a phase separation reagent may decrease the possibility of contaminating RNA with DNA.
 
Cat.No. BP 151
200 ml - $585

BCP Paper

PolyAcryl Carrier
PolyAcryl Carrier is a molecular biology grade solution of acryl polymer designed for use in the isolation of small amounts of RNA or DNA. PolyAcryl Carrier does not affect the activity of restrictases, reverse transcriptase, Taq polymerase, DNA polymerase, ligase or other enzymes used for nucleic acid analysis.
Cat.No. PC 152
5 ml - $540
REFERENCES
Chomczynski P. and Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem.,162,156-159.
Chomczynski P. 1993. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques, 15, 532-537. 
Chomczynski P., Bowser-Finn R. and Sabatini L. 1994. A reagent for the single-step isolation of viral RNA from human serum and biopsy samples. J. NIH Res., 6, 83.
US Patents 4,843,155 and 5,346,994.
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TRI Reagent for RNA Isolation (500 ml)
TRI Reagent is a complete and ready-to-use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin. (Cat# TR 118-500ML)
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TRI Reagent® RT

TRI Reagent® RT, 100 ml (Cat.#RT 111-100ML)

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TRI Reagent® RT

TRI Reagent® RT, 200 ml (Cat.# RT 111-200ML)

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TRI Reagent® RT (500 ml)
TRI Reagent® RT is an upgraded (patent pending) reagent for the single-step method of RNA isolation. Unlike the previous single-step methods, TRI Reagent® RT isolates RNA that is free of DNA contamination, as tested by 35-cycle PCR. Thus, no DNase treatment is necessary to use the isolated RNA in RT-PCR. (Cat# TR 118-500ML)
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BCP - Phase Separation Reagent
The Phase Separation Reagent consists of molecular biology grade 1-bromo-3-chloropropane (BCP). BCP replaces chloroform in the single-step method of RNA isolation or the simultaneous isolation of RNA, DNA and proteins. Substituting BCP for chloroform does not affect the quality of isolated RNA, DNA or proteins
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PolyAcryl Carrier for RNA & DNA Isolation
PolyAcryl Carrier is a molecular biology grade solution of acryl polymer designed for use in the isolation of small amounts of RNA or DNA. PolyAcryl Carrier does not affect the activity of restrictases, reverse transcriptase, Taq polymerase, DNA polymerase, ligase or other enzymes used for nucleic acid analysis. (Cat.# PC 152)
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TRI Reagent®LS
TRI Reagent®LS is a complete and ready to use reagent for the isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from liquid samples of human, animal, plant, yeast, bacterial and viral origin. (Cat.# TS 120-500ML)
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Precipitation Carrier
Precipitation Carrier is a molecular biology grade solution of acryl polymer designed for use in the isolation of small amounts of RNA (< 10 g). It can be used with both RNAzol® RT and TRI Reagent®. In the RNAzol RT procedure, Precipitation Carrier facilitates the recovery of both large (> 200 bases) and small (< 200 bases) RNA in separate fractions. This carrier does not affect the activity of restrictases, reverse transcriptase, Taq polymerase, ligase or other enzymes used for nucleic acid analysis. (Cat.# PC 173)
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