Quantibody® array is an array-based multiplex sandwich ELISA system for simultaneously quantitative measurement the concentration of multiple cytokines in the one sample. It takes the advantage of the high detection specificity / sensitivity of ELISA and the high throughput of the arrays. Like a traditional sandwich based ELISA, it uses a pair of cytokine specific antibodies for detection. By arraying multiple specific capture antibodies onto a glass support, multiplex detection of cytokines in one experiment is made possible.
For cytokine quantification, predetermined cytokine standards are provided, whose serial dilutions will be processed alongside the unknown samples. The signals will be detected using fluorescence-based detection method for consistency and reliability. By comparing signals from unknown samples to the standard curve, the unknown cytokine concentration in the samples will be determined.
Product Highlights • Detect the concentration of up to 160 human (120 mouse) cytokines in a single experiment • Proven Sandwich ELISA based technology platform • Similar or better detection sensitivity as ELISA, as low as 1 pg levels • High specificity and system reproducibility • Requires as little as 50 ul of sample • Get the results the same day (around 6 hr processing time) • Hundreds of samples can be processed by one technician in a single day. • Affordable and cost effective (Only a fraction of the ELISA cost) • Suitable for diverse sample types: serum, plasma, conditioned media and other body fluid. • User can choose own cytokine panels from pool of 400 cytokines, related proteins • Compatible with most of the laser scanners on the market. • Signal stability: The fluorescence signal on slides can retain for years • Quantitative analysis software available for one step computation
