Application Notes - ONWON TRADING LTD

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Product Catalogue
	Diagnostic Products
	Life Science Research Products
	Suppliers
		Millipore
		Cayman Chemical
		Viogene
		eBioscience
		Kapa Biosystems
			KAPA SYBR FAST qPCR Kits
			KAPA2G Robust HotStart
			KAPA Blood PCR Kits
			KAPA2G Fast HotStart
			KAPAHiFi HotStart DNA Polymerase
			KAPA2G Robust DNA Polymerase
			KAPAHiFi DNA Polymerase
			KAPA2G Fast DNA Polymerase
			KAPA PROBE FAST qPCR Kits
			Application Notes
				GC rich DNA
				Mouse Genotyping
				Colony PCR (Pick Clone)
				Sanger Sequencing
				Next-Generation DNA Sequencing
				Site-Directed Mutagenesis
				Gene Expression
				Multiplex PCR
				Single Protocol PCR
			Customer Testimonials
		CELLnTEC
		Cedarlane
		Trevigen
		Epigentek
		Bioworld
		Jena Bioscience
		Innova Biosciences
		Mirus Bio
		ECM Biosciences
		Immune Technology
		Invivoscribe Technologies
		Biotium
		Biocolor
		AdipoGen
		LifeSpan Biosciences
		Thermo Scientific (Pierce Antibodies)
		Everest Biotech

Application Notes

GC rich DNA

Amplification of GC rich DNA
(data kindly provided by Maryke Appel & Marelize Cruywagen (Kapa Biosystems, Cape Town, South Africa)

N/A

Mouse Genotyping

Mouse genotyping workflows can be significantly streamlined and turnaround times reduced from over 6 hours to 1-2 hours using KAPA2G polymerase.

N/A

Colony PCR (Pick Clone)

Unrivalled performance in colony PCR

N/A

Sanger Sequencing

Kapa Biosystems offers a range of engineered DNA polymerases for upstream DNA amplification and downstream finishing for Sanger DNA sequencing applications.

N/A

Next-Generation DNA Sequencing

From Sample Enrichment to Genome Finishing: Kapa Biosystems offers a range of products to improve your NGS workflow.

N/A

Site-Directed Mutagenesis

The world highest fidelity polymerase virtually eliminated clones containing spurious mutations.

N/A

Gene Expression

Real-time PCR (qPCR) is the preferred method for DNA and cDNA quantification because of its high sensitivity, reproducibility, and wide dynamic range [1].

N/A

Multiplex PCR

A challenging application that typically requires more optimization than standard, single amplicon PCR assays.

N/A

Single Protocol PCR

Consolidate cycling protocols, reaction conditions, and enzymes with Kapa Biosystems’ novel DNA polymerases.

N/A