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KAPAHiFi HotStart DNA Polymerase

A second-generation polymerase engineered for extreme fidelity and robustness - 100x fidelity improvement compared to Taq polymerase.


KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi DNA Polymerase results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g GC-rich) - standardize your high fidelity PCRs with a single enzyme.


The engineered KAPA HiFi DNA Polymerase plus optimized buffer system offers:



  • Superior fidelity - 100x improvement compared to Taq polymerase.
  • Robust performance across a wide range of difficult templates, including both AT- and GC-rich amplicons.
  • Consolidation of PCR protocols and reaction conditions with single enzyme.
  • Long range amplification of complex targets - up to 15 kb from genomic DNA.
  • High speed - reduce reaction times by up to 75%.
KAPA HiFi™ HotStart DNA Polymerase, with dNTPs Mix, 100 units
KAPA HiFi™ HotStart DNA Polymerase, with dNTPs Mix, 100 units (Cat.#KK2501)
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KAPA HiFi™ HotStart DNA Polymerase, with dNTPs Mix, 250 units
KAPA HiFi™ HotStart DNA Polymerase, with dNTPs Mix, 250 units ) (Cat.#KK2502)
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KAPAHiFi HotStart DNA Polymerase, with dNTPs mix, 20 units (sample)
KAPAHiFi HotStart DNA Polymerase, with dNTPs mix, 20 units (sample) (Cat.#KK2500)
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KAPA HiFi™ HotStart DNA Polymerase, Readymix (100 x 25 ul rxn)
KAPA HiFi™ HotStart DNA Polymerase, Readymix (100 x 25 ul rxn), 100 rxn (Cat.#KK2601)
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KAPA HiFi™ HotStart DNA Polymerase, Readymix (500 x 25 ul rxn)
KAPA HiFi™ HotStart DNA Polymerase, Readymix (500 x 25 ul rxn), 500 rxn (Cat.#KK2602)
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KAPAHiFi HotStart DNA Polymerase, ReadyMix (20 x 25 ul rxn), 20 rxn (sample)
KAPAHiFi HotStart DNA Polymerase, ReadyMix (20 x 25 ul rxn), 20 rxn (sample) (Cat.#KK2600)
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