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KAPA2G Fast DNA Polymerase
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KAPA2G Fast DNA Polymerase is a second-generation enzyme derived through a process of molecular evolution. The KAPA2G Fast DNA Polymerase was engineered for higher processivity and speed, offering significantly faster extension rates and improved PCR success rates than wild-type Taq polymerase. KAPA2G Fast is well suited for both routine and high-throughput PCR, achieving higher yields and improved sensitivity than competitor enzymes across a wide range of amplicon types. KAPA2G Fast DNA Polymerase and optimized buffer system offers: - Extension times as low as 1 sec per kilobase.
- Outstanding performance when compared to standard and hot start Taq polymerase.
- 20 - 70% reductions in total PCR time.
- No requirement for specialist PCR consumables or instrumentation.
Amplification of 15 human amplicons using KAPA2G Fast ReadyMix with dye or competitor ready-to-use PCR mixes containing Taq.
Reactions were set up and as described in Methods. Cycling protocols consisted of an initial denaturation of 3 min (95 °C), followed by 35 cycles of 15 sec denaturation (95 °C), 15 sec annealing (60 °C) and 15 sec extension (72 °C) for KAPA2G Fast ReadyMix with dye, or 30 sec denaturation (95 °C), 30 sec annealing (60 °C) and 1 min extension (72 °C) for competitor products. The total cycling time was 37 min and 1 h 21 for KAPA2G Fast ReadyMix with dye and competitors, respectively.
Amplification of a 327 bp human amplicon from a 5-fold serial dilution of human genomic DNA, using KAPA2G Fast ReadyMix with dye (fast cycling protocol) and competitor products (standard or fast cycling protocols).
Reactions were set up as described in Methods. The amount of template DNA in each reaction was as follows: 1 = 50 ng, 2 = 10 ng, 3 = 2 ng, 4 = 400 pg, 5 = 80 pg and 6 = 16 pg. Cycling protocols consisted of an initial denaturation of 3 min (95 °C), followed by 35 cycles of 15 sec denaturation (95 °C), 15 sec annealing (60 °C) and 5 sec extension (72 °C) for the KAPA2G Fast cycling protocol (top), or 30 sec denaturation (95 °C), 30 sec annealing (60 °C) and 1 min extension (72 °C) for the slow, wild-type enzyme protocol (bottom). The total cycling time was 48 min and 1 h 37 min for the fast and slow cycling protocols, respectively.
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