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Triple Cut Virus DNA plus Eufection Transfection Reagent BacVector DNA Kits are designed for efficient and reliable construction of baculovirus recombinants starting with pBAC™, pAcPIE1, or other compatible transfer plasmid DNA. The BacVector Triple Cut Virus DNA has been digested with a restriction enzyme that cuts within the essential gene ORF1629, upstream of the lacZ gene and polh promoter, and within the lacZ gene (1). BacVector Triple Cut Virus DNA thus has an extremely low non-recombinant background, and typically produces nearly 100% recombinant plaques. Kits contain the Insect GeneJuice?Transfection Reagent, BacVector Triple Cut Virus DNA and Transfection Control Plasmid.
Features
• Greater than 95% recombinants
• BacVector-2000 DNA engineered to remove nonessential genes
• BacVector-3000 DNA engineered to remove additional genes encoding proteolytic and degradative enzymes
• DNA Kits include Insect GeneJuice Transfection Reagent and Triple Cut Virus DNA
• Low cost per transfection
• Compatible with all pBAC plasmids and many other transfer vectors |
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