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BacMagic™ Transfection Kits
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| The BacMagic™ DNA Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. It improves on the traditional method for generating recombinant baculoviruses by eliminating the time-consuming steps of plaque purification. The BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted and a bacterial artificial chromosome (BAC) in place of the polyhedrin (polh) coding region. This combination prevents nonrecombinant virus from replicating in insect cells, yet allows the viral DNA to be propagated as a circular DNA in bacterial cells. The target coding sequence in a compatible transfer plasmid, such as the pBAC or pTriEx vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the virus ORF1629 while the target coding sequence replaces the BAC sequence. Only the recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic Transfection Kit contains the BacMagic DNA, Insect GeneJuice Transfection Reagent, Sf9 cells, BacVector Medium, and a positive control plasmid, all of which have been qualified for optimal transfection performance. |
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